|Carine Kronland-Martinet – Chair
Works as: PhD. Student at the Center for Particle Physics of Marseille (CPPM, Marseille) and the Developmental Biology Institute, IBDM, of Marseille.
Works on: Development of intra-vital spectral X-ray imaging in a tumor context
I recently finished my PhD. thesis. The subject was based on a collaboration between a laboratory specialized in particle physics (Center for Particle Physics, CPPM, imXgam team, Marseille) and two laboratories specialized in biology (the Developmental Biology Institute, IBDM, of Marseille and the Neuroscience Institute of Timone, INT, imaPath team, Marseille) under the supervision of Pr. Christian Morel and Dr. Franck Debarbieux. I worked on the development of K-edge (spectral) intra-vital X-ray tomography with the hybrid pixel camera XPAD3 incorporated in micro-CT PIXSCAN II. The main objective of my thesis was to use new technologies offered by the X-rays camera XPAD3 to make it possible to effectuate imaging of the whole body of small, live animals in a tumor context to visualize the vascularization and inflammation independently. To reach this goal I used the idea of composite pixels that make K-edge imaging possible on live animals without movement artifacts. This new approach also enables to decrease the acquisition time and the radiation dose by three with a compromise on the resolution. In this context I developed a way to mark cells of the immune systems with nanoparticles (bimodal nanoparticles visible on X-ray and on optical fluorescence) to make them visible under X-rays in the body of a small animal. To validate this novel approach I used in addition fluorescence optics and X-rays.
|Jonathan Vigne – Co-chair
Works on: In vivo nuclear molecular imaging of serine proteases
I did my master thesis at Cyceron imaging platform, Caen, France. Under the supervision of Pr A. Manrique, I worked on the development of innovative in vivo imaging approaches for the detection of post-myocardial infarction neo-angiogenesis. The main objective of my thesis was to functionalize a polymeric formulation of cyclodextrins nanoparticules using radiolabeled RGD peptides. These peptides are known to bind integrins used as biomarkers of neo-angiogenesis. To enhance their pharmacokinetic profile (biological half-life, furtivity), we developed inclusion complexes between radiolabeled RGD peptides and cyclodextrins tetrapolymers (α, β, γ) and we assessed its neo-angiogenesis detection pattern on a preclinical model of myocardial ischemia-reperfusion.
I started my PhD. thesis in the team of Pr D. Le Guludec (INSERM U1148). We aim to develop radiolabeled ligands to perform in vivo nuclear imaging of serine proteases activity. SPECT and PET modalities have been chosen because of their sensitivity and suitability for in depth in vivo imaging. Monitoring of serine protease activity would provide a useful tool in the diagnostic and management of different diseases such as endocarditis, stroke, aortic and intracranial aneurysms.
|Biliana Todorova – Secretary
Works as: Postdoctoral researcher in the Unit of Antibodies in Therapy and Pathology at Institut Pasteur in Paris
Works on: In vivo imaging of the anaphylactic reaction in preclinical models
I did my PhD thesis in the Immuno-Virology Division at the Atomic Energy Commission (CEA) in France. Under the supervision of Dr Roger Le Grand and Dr Catherine Chapon, I worked on the development of innovative in vivo imaging approaches for the visualization of the immune cell behavior in non-human primates. The main objective of my thesis was to describe early immune events that occur in the skin, after DNA vaccination associated to local electroporation. To reach our goal, we used fiber-based confocal microscopy to image cutaneous dendritic cells in a noninvasive manner and we performed time-lapse laser-scanning confocal microscopy on skin biopsies to investigate the dynamic parameters of cell mobility and interactions. In addition an MRI approach was used to visualize the popliteal lymph node after intradermal injection of iron oxide nanoparticles.
I started my postdoc in the team of Dr Pierre Bruhns, which have recently highlighted the mechanism of induction of the active anaphylaxis in the mouse involving the IgG receptors, the neutrophils and the platelet activating factor (PAF). We aim to develop in vivo imaging approaches in order to visualize anaphylactic reactions in real time. Thus whole body bioluminescence and fluorescence imaging will be associated to intravital microscopy to study the behavior of various immune cell populations during the allergic shock.